We propose to generate a vascular replacement based on complete reconstitution by using three vascular components (endothelial cells, smooth muscle cells, and pericytes) together with biodegradable scaffolds with topological characteristics similar to native vasculature. We will explore the processes needed to construct the conduit with defined vascular components and we will determine the conditions under which these components can resume function under experimental manipulation. If we are successful, we can produce a novel biologic product that yields a living vascular replacement with responsiveness to environmental stimuli, with potential to grow according to local regulation, and with sufficient mechanical strength and readiness for suturing; these replacements will be nonthrombogenic and self-repairing. If adequate cell sources are located, our proposed process will reduce both the time and the cost of manufacturing. Following GMP regulations, we will be able to make consistently useable products that can be stored as off-the-shelf medical biologicals and used in emergencies.
